nonlinear regression and one-site binding model Search Results


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Binding of F30 and F124 2′F-Py RNA aptamers on different F-type and R-type α-Syn fibrillar polymorphs compared to DNA aptamers previously described in the literature. ( A ) example of binding curves of F30 and F124 against F-type (upper panel) and R-type (lower panel) α-Syn fibrillar polymorphs. The binding of aptamers are in red while the binding of a scramble sequence was used to evaluate the <t>nonspecific</t> binding (black dotted curves). The binding curves were fit using the model ‘One site - Fit total and nonspecific binding’ from GraphPad Prism 9 to calculate the apparent K d of the interaction. ( B ) Apparent K d s of 2′F-Py RNA and DNA aptamers on different F-type and R-type α-Syn fibrillar polymorphs. The values presented in the table represent the average of the apparent K d s and their standard deviation calculated from completely independent experiments (in triplicate and duplicate for 2′F-Py RNA and DNA aptamers, respectively).
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Binding of F30 and F124 2′F-Py RNA aptamers on different F-type and R-type α-Syn fibrillar polymorphs compared to DNA aptamers previously described in the literature. ( A ) example of binding curves of F30 and F124 against F-type (upper panel) and R-type (lower panel) α-Syn fibrillar polymorphs. The binding of aptamers are in red while the binding of a scramble sequence was used to evaluate the <t>nonspecific</t> binding (black dotted curves). The binding curves were fit using the model ‘One site - Fit total and nonspecific binding’ from GraphPad Prism 9 to calculate the apparent K d of the interaction. ( B ) Apparent K d s of 2′F-Py RNA and DNA aptamers on different F-type and R-type α-Syn fibrillar polymorphs. The values presented in the table represent the average of the apparent K d s and their standard deviation calculated from completely independent experiments (in triplicate and duplicate for 2′F-Py RNA and DNA aptamers, respectively).
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Binding of F30 and F124 2′F-Py RNA aptamers on different F-type and R-type α-Syn fibrillar polymorphs compared to DNA aptamers previously described in the literature. ( A ) example of binding curves of F30 and F124 against F-type (upper panel) and R-type (lower panel) α-Syn fibrillar polymorphs. The binding of aptamers are in red while the binding of a scramble sequence was used to evaluate the <t>nonspecific</t> binding (black dotted curves). The binding curves were fit using the model ‘One site - Fit total and nonspecific binding’ from GraphPad Prism 9 to calculate the apparent K d of the interaction. ( B ) Apparent K d s of 2′F-Py RNA and DNA aptamers on different F-type and R-type α-Syn fibrillar polymorphs. The values presented in the table represent the average of the apparent K d s and their standard deviation calculated from completely independent experiments (in triplicate and duplicate for 2′F-Py RNA and DNA aptamers, respectively).
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Binding of F30 and F124 2′F-Py RNA aptamers on different F-type and R-type α-Syn fibrillar polymorphs compared to DNA aptamers previously described in the literature. ( A ) example of binding curves of F30 and F124 against F-type (upper panel) and R-type (lower panel) α-Syn fibrillar polymorphs. The binding of aptamers are in red while the binding of a scramble sequence was used to evaluate the <t>nonspecific</t> binding (black dotted curves). The binding curves were fit using the model ‘One site - Fit total and nonspecific binding’ from GraphPad Prism 9 to calculate the apparent K d of the interaction. ( B ) Apparent K d s of 2′F-Py RNA and DNA aptamers on different F-type and R-type α-Syn fibrillar polymorphs. The values presented in the table represent the average of the apparent K d s and their standard deviation calculated from completely independent experiments (in triplicate and duplicate for 2′F-Py RNA and DNA aptamers, respectively).
One Site Binding Linear Regression Model Prism 5.0, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Binding of F30 and F124 2′F-Py RNA aptamers on different F-type and R-type α-Syn fibrillar polymorphs compared to DNA aptamers previously described in the literature. ( A ) example of binding curves of F30 and F124 against F-type (upper panel) and R-type (lower panel) α-Syn fibrillar polymorphs. The binding of aptamers are in red while the binding of a scramble sequence was used to evaluate the <t>nonspecific</t> binding (black dotted curves). The binding curves were fit using the model ‘One site - Fit total and nonspecific binding’ from GraphPad Prism 9 to calculate the apparent K d of the interaction. ( B ) Apparent K d s of 2′F-Py RNA and DNA aptamers on different F-type and R-type α-Syn fibrillar polymorphs. The values presented in the table represent the average of the apparent K d s and their standard deviation calculated from completely independent experiments (in triplicate and duplicate for 2′F-Py RNA and DNA aptamers, respectively).
Nonlinear Regression Analyses Of One Site Binding Model Concentration Response Experimental Data Using Graphpad Prism 3.0, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Binding of F30 and F124 2′F-Py RNA aptamers on different F-type and R-type α-Syn fibrillar polymorphs compared to DNA aptamers previously described in the literature. ( A ) example of binding curves of F30 and F124 against F-type (upper panel) and R-type (lower panel) α-Syn fibrillar polymorphs. The binding of aptamers are in red while the binding of a scramble sequence was used to evaluate the <t>nonspecific</t> binding (black dotted curves). The binding curves were fit using the model ‘One site - Fit total and nonspecific binding’ from GraphPad Prism 9 to calculate the apparent K d of the interaction. ( B ) Apparent K d s of 2′F-Py RNA and DNA aptamers on different F-type and R-type α-Syn fibrillar polymorphs. The values presented in the table represent the average of the apparent K d s and their standard deviation calculated from completely independent experiments (in triplicate and duplicate for 2′F-Py RNA and DNA aptamers, respectively).
Competitive Binding One Site Fit Ki Non Linear Regression Analysis Model Graphpad Prism 5, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Binding of F30 and F124 2′F-Py RNA aptamers on different F-type and R-type α-Syn fibrillar polymorphs compared to DNA aptamers previously described in the literature. ( A ) example of binding curves of F30 and F124 against F-type (upper panel) and R-type (lower panel) α-Syn fibrillar polymorphs. The binding of aptamers are in red while the binding of a scramble sequence was used to evaluate the <t>nonspecific</t> binding (black dotted curves). The binding curves were fit using the model ‘One site - Fit total and nonspecific binding’ from GraphPad Prism 9 to calculate the apparent K d of the interaction. ( B ) Apparent K d s of 2′F-Py RNA and DNA aptamers on different F-type and R-type α-Syn fibrillar polymorphs. The values presented in the table represent the average of the apparent K d s and their standard deviation calculated from completely independent experiments (in triplicate and duplicate for 2′F-Py RNA and DNA aptamers, respectively).
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Binding of F30 and F124 2′F-Py RNA aptamers on different F-type and R-type α-Syn fibrillar polymorphs compared to DNA aptamers previously described in the literature. ( A ) example of binding curves of F30 and F124 against F-type (upper panel) and R-type (lower panel) α-Syn fibrillar polymorphs. The binding of aptamers are in red while the binding of a scramble sequence was used to evaluate the <t>nonspecific</t> binding (black dotted curves). The binding curves were fit using the model ‘One site - Fit total and nonspecific binding’ from GraphPad Prism 9 to calculate the apparent K d of the interaction. ( B ) Apparent K d s of 2′F-Py RNA and DNA aptamers on different F-type and R-type α-Syn fibrillar polymorphs. The values presented in the table represent the average of the apparent K d s and their standard deviation calculated from completely independent experiments (in triplicate and duplicate for 2′F-Py RNA and DNA aptamers, respectively).
Nonlinear One Site Specific Binding With Hill Slope Model Fitted By Graphpad Prism Version 6.07 For Windows, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Binding of F30 and F124 2′F-Py RNA aptamers on different F-type and R-type α-Syn fibrillar polymorphs compared to DNA aptamers previously described in the literature. ( A ) example of binding curves of F30 and F124 against F-type (upper panel) and R-type (lower panel) α-Syn fibrillar polymorphs. The binding of aptamers are in red while the binding of a scramble sequence was used to evaluate the nonspecific binding (black dotted curves). The binding curves were fit using the model ‘One site - Fit total and nonspecific binding’ from GraphPad Prism 9 to calculate the apparent K d of the interaction. ( B ) Apparent K d s of 2′F-Py RNA and DNA aptamers on different F-type and R-type α-Syn fibrillar polymorphs. The values presented in the table represent the average of the apparent K d s and their standard deviation calculated from completely independent experiments (in triplicate and duplicate for 2′F-Py RNA and DNA aptamers, respectively).

Journal: Nucleic Acids Research

Article Title: Aptamer binding footprints discriminate α-synuclein fibrillar polymorphs from different synucleinopathies

doi: 10.1093/nar/gkae544

Figure Lengend Snippet: Binding of F30 and F124 2′F-Py RNA aptamers on different F-type and R-type α-Syn fibrillar polymorphs compared to DNA aptamers previously described in the literature. ( A ) example of binding curves of F30 and F124 against F-type (upper panel) and R-type (lower panel) α-Syn fibrillar polymorphs. The binding of aptamers are in red while the binding of a scramble sequence was used to evaluate the nonspecific binding (black dotted curves). The binding curves were fit using the model ‘One site - Fit total and nonspecific binding’ from GraphPad Prism 9 to calculate the apparent K d of the interaction. ( B ) Apparent K d s of 2′F-Py RNA and DNA aptamers on different F-type and R-type α-Syn fibrillar polymorphs. The values presented in the table represent the average of the apparent K d s and their standard deviation calculated from completely independent experiments (in triplicate and duplicate for 2′F-Py RNA and DNA aptamers, respectively).

Article Snippet: The apparent K d values were determined by fitting binding curves using the model ‘One site—Fit total and nonspecific binding’ from GraphPad Prism 10 considering the binding of the scramble sequence as non-specific binding.

Techniques: Binding Assay, Sequencing, Standard Deviation